State of the art in circulating miR-95 as a new candidate for diagnostic in Human Chronic Lymphocytic Leukemia

 Backgrounds: MicroRNAs (miRNAs) have crucial roles in cellular and molecular processes related to different malignancies including chronic lymphocytic leukemia (CLL). In recent years the most studies demonstrated that the expression of miR-95, alter in several diseases. Long non-coding RNAs (lncRNAs) a heterogeneous group of non-coding and regulatory RNAs. The present study investigated the association of miR-95 mRNA expression with CLL by quantitative real-time PCR for the first time. Methods: Sixty samples, including 30 CLL, and 30 healthy controls were sampled during a period of 4 months. The expression of miR-95 was determined by quantitative real-time PCR in peripheral blood mononuclear cells (PBMCs) of CLL patients in comparison with healthy subjects. Also, in silico pathway enrichment analysis performed on validated and predicted targets of miR-95 in several databases including miRecords and miRTarBase and predicted putative lncRNAs interplay with genes and miRNAs expression by mirwalk. Results: The expression of miR-95 was found to be significantly reduced in CLL patients compared with healthy controls (P <0.005). Conclusion: Our findings suggested that miR-95 probably could be used as a new biomarker for early diagnosis of CLL patients, at least in Iranian patients. LncRNAs play a significant role in regulating cellular evolution, differentiation, and other processes. LncRNAs may serve as important regulators in tumorigenesis.